Journal: Cell Death & Disease

Article Title: Erythropoietin decreases apoptosis and promotes Schwann cell repair and phagocytosis following nerve crush injury in mice

doi: 10.1038/s41419-025-07825-4

Figure Lengend Snippet: A , B Representative IF images and quantitative results of repair SCs (anti-p75 staining) phagocytosis of myelin debris (anti-MPZ staining) in saline and EPO treated nerve tissues on post-SNCI days 3, 5, and 7. n = 5/group. C , D Representative IF images and quantitative results of repair SCs (phalloidin staining) phagocytosis of myelin debris (PKH26 staining) following 24 h EPO (10 IU/mL) treatment under LPS (500 ng/mL) stress conditions. n = 3/group. E , F Flow cytometry images and quantitative results of repair SCs (p75 positive cells) phagocytosis of myelin debris (PKH26 staining) following 24 h EPO (10 IU/mL) treatment under LPS (500 ng/mL) stress conditions. n = 3/group. Data are represented as mean ± SEM. The statistical significance is indicated by asterisks (**P < 0.0021, ***P < 0.0002, and ****P < 0.0001 vs. saline group) and compared using two-tailed, unpaired t-tests or ordinary one-way ANOVA.

Article Snippet: Next, primary antibody staining was performed using p75 (1:100, # ab1554, Millipore Sigma), MPZ (1:100, # PZO, Aveslabs), Ki67 (1:100, # 9129, Cell Signaling), and c-Jun (1:100, # Sc74543, Santa Cruz) with an overnight incubation at 4 °C.

Techniques: Staining, Saline, Flow Cytometry, Two Tailed Test

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Selective RNAi silencing of Schwann cell Piezo1 alleviates mechanical hypersensitization following peripheral nerve injury

doi: 10.1016/j.omtm.2025.101433

Figure Lengend Snippet: GFP expression after intrasciatic AAVolig001-CBA-GFP (A–D) GFP expression in the nerve fascicles (dashed circles) of cross-sectioned sciatic nerves (A and B). Detection of GFP signals in longitudinal sections of tibial and sural serves (C and D). (E–J) Representative IHC images illustrate selective GFP expression in both mSCs and nmSCs, colabeled with SCs markers MPZ, MBP, GAP43, and P75NTR (E–H). Empty and white arrowheads in (F) and (H) point to mSCs and nmSCs, respectively; no GFP signals in the afferent axons labeled by Tubb3 and NF200 (I and J). Scale bars (μm): (A) and (B), 100; (C), 500; (D)–(F), 100. Antibodies for double labeling are indicated in each montage IHC image. Scales: 50 μm for all. GAP43, growth-associated protein 43; MBP, myelin basic protein; MPZ, myelin protein zero; NF200, neurofilament 200; P75NTR, p75 neurotrophin receptor; and Tubb3, β3-tubulin.

Article Snippet: Cells and tissue sections were immunolabeled with the selected primary antibodies mouse Piezo1 (1:400, Novus Biologicals, NBP2-75617), rabbit GFP (1:500, Cell Signaling Technology [Danvers, MA], 2555S), rabbit mCherry (1:400, Cell Signaling Technology, 43590), rabbit GAP43 (1:400, Cell Signaling Technology, 8945), rabbit p75NTR (1:400, Cell Signaling Technology, 8238), rabbit MPZ (1:1,000, Cell Signaling Technology, 57518s), rabbit S100 (1:1,000, Cell Signaling Technology, 13018), goat MBP (1:1,000, Santa Cruz Biotechnology [SCB], sc13912), goat Sox10 (1:200, SCB, sc17342), rabbit NF200 (1:1,000, Cell Signaling Technology, 30564), rabbit Tubb3 (1:1,000, Cell Signaling Technology, 5586), mouse cytokeratin14 (CK14, 1:200, SCB, sc53253), and rabbit glial fibrillary acidic protein (1:1,000, Dako [Glostrup, Denmark, Z0334) in a humid atmosphere overnight at 4°C.

Techniques: Expressing, Labeling